Table 2.
Proteins identified in sea bream kidney tissue.
| N. | Protein | Acc. no. | Species | Pred. mass | Pred. pI | Sc. | QM (%c.) | FC | p |
|---|---|---|---|---|---|---|---|---|---|
| 1 | Alanine aminotransferase, mt | gi|37783307 | S. aurata | 62091 | 8.54 | 527 | 26(23) | 2.3 | 0.0016 |
| 2 | Aldehyde dehydrogenase, mt | gi|118503 | E. caballus | 54532 | 5.7 | 76 | 2(4) | 3.3 | 0.0005 |
| 3 | Dihydrodipicolinate synthase, mt | gi|47208001 | T. nigroviridis | 34365 | 8.17 | 100 | 9(5) | 1.6 | 0.0001 |
| 4 | Methylmalonate semialdehyde dehydrogenase, mt | gi|47230188 | T. nigroviridis | 57397 | 7.06 | 410 | 10(12) | 2.3 | 0.0005 |
| 5 | Dihydrodipicolinate synthase, mt | gi|47208001 | T. nigroviridis | 34365 | 8.17 | 110 | 9(6) | 2.2 | 0.0006 |
| 6 | ATP synthase, beta subunit, mt | gi|47605558 | C. carpio | 55327 | 5.05 | 1432 | 76(46) | 1.5 | 0.005 |
| 7 | S-adenosyl-homocysteine hydrolase | gi|178277 | H. sapiens | 48254 | 6.03 | 156 | 7(6) | 2.6 | 0.0104 |
| 8 | Phosphoenolpyruvate carboxykin. | gi|24637098 | S. aurata | 14574 | 5.61 | 48 | 1(9) | <1.5 | >0.05 |
| 9 | Antiquitin | gi|61742178 | A. schlegelii | 55832 | 5.88 | 369 | 9(13) | 1.71 | >0.05 |
| 10 | Wap65 | gi|119393859 | A. schlegelii | 49162 | 5.40 | 250 | 11(10) | 1.86 | >0.05 |
| 11 | Transferrin | gi|34329603 | A. schlegelii | 76152 | 6.38 | 418 | 12(9) | 2.69 | >0.05 |
| 12 | Transferrin | gi|33113484 | P. major | 76146 | 5.72 | 206 | 6(6) | <1.5 | >0.05 |
| 13 | Beta actin | gi|33526989 | M. albus | 42110 | 5.31 | 752 | 41(45) | <1.5 | >0.05 |
| 14 | ATP synthase, mt | gi|66773080 | D. rerio | 55080 | 5.25 | 1546 | 79(47) | <1.5 | >0.05 |
| 15 | Cytoplasmic actin | gi|13699190 | L. japonicum | 42137 | 5.30 | 777 | 57(42) | <1.5 | >0.05 |
| 16 | Beta actin | gi|49868 | M. musculus | 39446 | 5.78 | 557 | 59(28) | <1.5 | >0.05 |
| 17 | Acyl-Co A dehydrogenase, mt | gi|47209002 | T. negroviridis | 39802 | 6.08 | 211 | 4 (11) | 1.9 | 0.0117 |
| 18 | Fructose-biphosphate aldolase B | gi|1703243 | S. aurata | 40190 | 8.43 | 664 | 25(20) | <1.5 | >0.05 |
| 19 | Electron transfer flavopr. alpha, mt | gi|47225813 | T. nigroviridis | 35017 | 7.64 | 406 | 14(26) | <1.5 | >0.05 |
| 20 | PEBP superfamily | gi|47221502 | T. nigroviridis | 21069 | 6.89 | 393 | 20(27) | <1.5 | >0.05 |
| 21 | Carbonic anhydrase | gi|56554783 | P. americanus | 28512 | 5.22 | 64 | 4(10) | 2.21 | >0.05 |
| 22 | Peroxiredoxin | gi|47220267 | T. nigroviridis | 22280 | 5.44 | 313 | 24(22) | 2.5 | 0.0025 |
| 23 | Peroxiredoxin family protein | gi|93211500 | P. maxima | 22063 | 5.58 | 364 | 21(32) | 1.6 | 0.05 |
| 24 | Enoyl-CoA hydratase short chain | gi|12805413 | M. musculus | 31636 | 8.51 | 224 | 13(12) | <1.5 | >0.05 |
| 25 | Glutathione S-transferase | gi|34014736 | S. aurata | 24748 | 8.51 | 205 | 10(28) | 1.6 | >0.05 |
| 26 | Nucleoside diphosphate kinase | gi|10121713 | G. mirabilis | 17214 | 8.52 | 299 | 39(48) | <1.5 | >0.05 |
| 27 | Cu/Zn superoxide dismutase | gi|62550923 | S. aurata | 6979 | 5.41 | 545 | 25(68) | 1.8 | >0.05 |
| 28 | Alpha-2 globin | gi|99122203 | S. aurata | 15887 | 8.79 | 96 | 11(6) | <1.5 | >0.05 |
| 29 | Beta globin | gi|91260232 | S. aurata | 16308 | 7.82 | 158 | 7(18) | <1.5 | >0.05 |
| 30 | Alpha-2 globin | gi|99122203 | S. aurata | 15887 | 8.79 | 466 | 23(41) | <1.5 | >0.05 |
| 31 | Beta globin | gi|91260232 | S. aurata | 16308 | 7.82 | 367 | 10(47) | <1.5 | >0.05 |
N, spot number; Acc. no., accession number; Pred. mass, predicted mass; Pred. pI, predicted pI; Sc, score; QM (%c.), Queries matched (% coverage); FC, fold change.
Protein identifications were performed both by MALDI-MS and nano-HPLC-nano-ESI-Q-TOF-MS/MS. Proteins with fold change > 1.5 and P < 0.05 are in bold. Proteins with fold change > 1.5 and p > 0.05 are in italics.