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. 2010 Oct 14;3:99. doi: 10.1186/1756-3305-3-99

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Copyright ©2010 Johnston et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of recombinant LspA proteins in T7 express E. coli with and without IPTG induction. wBmLspA and EcLspA transformed E. coli cell lysates (~ 200 μg total protein/well) were loaded. The expressed recombinant LspA proteins were detected with Mouse anti-His monoclonal antibody (details in methods and materials). For each construct of samples without (w/o) and with (w/) IPTG induction are shown, Lane 1 & 2: E. coli cell/pET21a (NC1), Lane 3 & 4: E. coli cell/pET21a-EcLspA.His (PC), Lane 5 & 6: E. coli cell/pET21a-wBmLspA.His (T1), Lane 7 & 8: E. coli cell/pGEX5.1 (NC2), Lane 9 & 10: E. coli cell/pGEX5.1-GST.wBmLspA.His (T2). The predicted molecular weights of recombinant wBmLspA.His, EcLspA.His and GST.wBmLspA.His proteins are 19, 20 and 44 kDa, respectively.