Table 1.

Complementation assays in E. coli ts LspA mutant Y815

E. coli Y815 LspA ts transformants	30°C	42°C
w/pET21a only (NC1)	+	-
w/pET21a-EcLspA.His (PC)	+	+
w/pET21a-wBmLspA.His (T1)	+	+
w/pGEX5.1 only (NC2)	+	-
w/pGEX5.1-GST.wBmLspA.His (T2)	+	-

Actively growing transformants were plated onto LB agar (100 μg/ml ampicillin, 10 μg/ml tetracycline and 0.6 mM IPTG) and incubated at both the permissive (30°C) and the nonpermissive (42°C) temperature for 3 days for colony formation as described [21]. '+' means growth, '-' means no growth