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. 2010 Oct 16;10:264. doi: 10.1186/1471-2180-10-264

Figure 4.

Figure 4

Verification of the expression of small RNA RyhB by RT-PCR. L: DNA ladder; 1. PCR amplification of S. oneidensis RNA without reverse transcription; 2. PCR amplification of sample after reverse transcription of RNA. The presence of the ~119 bp PCR product validates the expression of RyhB RNA. 3 and 4: PCR on two control intergenic regions (Chr. 367734-367820 and 796545-796665). The absence of PCR products indicates that genomic DNA has been completely removed from the RNA templates used for RT-PCR.