Table 2.
Oligonucleotide primers used in this study.
| Primer name | Sequence |
|---|---|
| strain construction | |
| fur-F1 | GGTCGACCAAGAGATTAGCAATGACAGATG |
| fur-R1 | GGAATTCGAGCAAGCTTATTCGTCGT |
| ryhB-F1 | GGTCGACAGGAGGAACTCTGATGACTGGTAATCTG |
| ryhB-R1 | GGAATTCAGTTAAATGTGGCGCAAAC |
| Reverse Transcription-PCR | |
| ryhB-F2 | TCTGACGTTGTTAAAGTGCTCC |
| ryhB-R2 | CCTAATGCGCCTATTCGCT |
| Control 1-F | TCAGGTTGTTTGGTATTGTGC |
| Control 1-R | CCATCAATCAAGGTTGTCG |
| Control 2-F | CTGTCAAATGGTGTGCTGC |
| Control 2-R | GTGTAACAGTGCTAAAGCCTGC |
| Control 3-F | TCTACTCAAATGACGAGCTGC |
| Control 3-R | GAAAAGCCGCCAAATGC |
| Control 4-F | TATGGTTTCCCGCTTTCG |
| Control 4-R | AACGCATCAGTGCTATTTGC |
| Control 5-F | TCACTCACAGAACGCTTCG |
| Control 5-R | GCAGCTACAGAATGTCACTACG |
| Control 6-F | TCTAGCAGGGATTAAATGAGC |
| Control 6-R | CCTTCGCCTTGTCTAAAGC |
| 5'- and 3'-RACE assays | |
| 5'- RNA adapter | GAUAUGCGCGAAUUCCUGUAGAACGAACACUAGAAGAAA |
| ryhB-R3 | AGAGTGTGTGAGCAATGTCG |
| 3'- RNA adapter | UUCACU GUUCUUAGCGGCCGCAUGCUC-idT |
| Quantitative RT-PCR | |
| RyhB-F | TCTGACGTTGTTAAAGTGCTCC |
| RyhB-R | CCTAATGCGCCTATTCGCT |
| SdhA-F | GAGCAGTTAAAAGCCATCC |
| SdhA-R | GTTGTCCAATTCTAAACACTCG |
| AcnA-F | ACCAACAAACGCTAGACTACC |
| AcnA-R | ATCATCGCTCCACAAACC |
| SodB-F | TCTACTGGAACTGCTTAGCACC |
| SodB-R | TGAATGCATCGAATGAACC |
| RecA-F | AACCCAGAAACCACAACG |
| RecA-R | ACCAACCACCTCATCACC |
Primer sequences were derived from the S. oneidensis MR-1 genome sequence [25]. F and R stand for forward and reverse primers, respectively.