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Histogram showing intracellular ROS expression in RGCs following CoCl2 treatment or 1% O2 exposure. Cells were treated with different concentrations of cobalt chloride (50, 100, 200 or 400 μM) (A) or exposed to1% oxygen (B) for a period of 24, 48 or 72 h. ROS were measured with H2-DCFH-DA dye. Results are means ± SE of 3 experiments. *p < 0.05
