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. 2010 Nov 1;24(21):2440–2450. doi: 10.1101/gad.604610

Figure 2.

Figure 2.

The N-terminal region of SMG6 confers binding to NMD factors and EJC components. (A–F) Interaction of V5-tagged SMG6 (wild type or deletion mutant) with HA-tagged NMD factors or endogenous Y14 and Magoh in human cells. Proteins were immunoprecipitated using anti-V5 antibodies. A V5-tagged MBP served as a negative control. The presence of endogenous Y14 and Magoh in the immunoprecipitates was analyzed by Western blotting using specific antibodies. In all panels, the protein bands observed at ∼55 kDa represent SMG6 degradation products comigrating with the immunoglobulin heavy chain that cross-react with the anti-V5 antibody.