Figure 7.

SMG6 interaction with the EJC is required for NMD. (A–D) Complementation assays were performed with wild-type SMG6 and various SMG6 mutants and the indicated NMD reporters, as described in Figure 1. An SMG6 protein carrying mutations in catalytic residues of the PIN domain and MBP served as controls. A and C show Northern blot analysis of representative RNA samples. Wild-type and PTC-containing mRNA levels were normalized to those of the GFP-NXF1 mRNA. For each condition, the normalized values of the wild-type reporter were set to 100 (white bar in B). Bshows mean values and standard deviation obtained in three independent experiments using the TCR-β reporter. The corresponding values for the β-globin reporter are shown in italics below C. (D) Western blotting showing the expression levels of recombinant proteins.