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. Author manuscript; available in PMC: 2011 Sep 24.
Published in final edited form as: FEBS Lett. 2010 Sep 4;584(18):4115–4120. doi: 10.1016/j.febslet.2010.08.040

Figure 3. MiR-24 direct targeting DND1 mRNA.

Figure 3

(A) The predicted miR-24 targeting sequence located in the 3’-untranslated region (3’-UTR) of DND1 mRNA. The seed region is highlighted in yellow. (B) The alignment of the miR-24 targeting sequences located in the 3’-UTR of the DND1 genes from 23 organisms. The evolutionarily conserved nucleotides are indicated with capital letters in the sequence shown on the bottom. Dual luciferase reporter assays were performed as described in the Materials and Methods section on cells transfected with constructs containing the predicted targeting sequence (pGL-DND1) or mutated targeting sequence (pGL-DND1m) cloned into the 3’-UTR of the reporter gene, and treated with anti-miR-24 LNA or scramble LNA (C), or treated with miR-24 mimic or control mimic (D). * indicates p < 0.05. Western blot analyses were performed to examine the effects of miR-24 on DND1 expression in UM2 cells that were treated with anti-miR-24 LNA or scramble LNA (E), or on cells that were treated with miR-24 mimic or control mimic (F). The expression of CDKN2A, a known target of miR-24 [21], was also examined in these cells. Data represents at least 3 independent experiments with similar results.