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. 2010 Oct 1;120(11):4077–4090. doi: 10.1172/JCI42758

Figure 4. Transcription factors involved in effector molecule production are highly upregulated in HCMV-specific cells.

Figure 4

(A) HCMV-specific cells during a primary response were stained by tetramers for 2 epitopes of HCMV, IE and pp65, and EBV BMLF1 as a control. In latency, an influenza (FLU) tetramer and the pp65 tetramer, respectively, were used to visualize memory cells that remain after the infection is cleared. EBV-specific cells were stained by the BMLF1 tetramer. Numbers depict the percentage of CD8+ cells or tetramer+ cells within the CD8+ population. Histograms visualize T-bet measured in the tetramer+ samples and (C) in total effector-type (CD8+CD45RA+CD27–) and memory-type (CD8+CD45RA–CD27+) cells in latency. Shown is 1 representative patient of 3 and 1 representative healthy donor of 4 analyzed. (B) During latency, total effector and memory cells were sorted, and quantitative PCR was performed to measure transcription factors T-bet, Eomes, and Blimp-1. Graphs represent mean and SD of 3 donors analyzed. Filled gray histograms denote staining of naive CD8+ T cells.