GlyRα3 immunoreactivity (GlyRα3-IR) showed almost-ubiquitous expression in the brainstem. Although there was no coexpression of GlyRα3 in motor nuclei like the facial nucleus (VII; A) and hypoglossal nucleus (XII; D) with GlyT2-eGFP neurons, GlyRα3 was expressed on glycinergic neurons within the BötC (B), pre-BötC (C), and spinal trigeminal nucleus (Sp5; E). Higher-magnification views of the merged images are shown at right. Scale bars: 200 μm (A and D); 100 μm (B, C, and E); 50 μm (higher magnification). AP, area postrema; IOPr, principal nucleus of the inferior olive; NA, nucleus ambiguus; NTS, nucleus of the solitary tract; RVLM, rostral ventrolateral medulla.