Figure 9. Recognition of KDR-expressing primary human cells by KDR-CAR–transduced PBLs.

(A) KDR expression on various normal primary human cells. The indicated human cell types were stained with PE-labeled mouse anti-human KDR antibody or isotype control antibody and analyzed by FACS. The filled histograms indicate KDR-specific staining; the open histograms indicate staining with isotype control antibody. (B) Primary human T cells were mock transduced or retrovirally engineered to express KDR1121-hCD828BBZ CAR and, 8 days later, cocultured with indicated human cells for 24 hours. Culture supernatants were assayed for IFN-γ by ELISA. Results are presented as mean ± SEM of triplicates. (C) The cytotoxicity of genetically engineered primary human T cells expressing KDR1121-hCD828BBZ or KDR1121-hCD28Z CAR against indicated target cells was determined by using a standard Cr⁵¹ release assay. Each data point reflects the mean of triplicates.