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. 2010 Oct 11;120(11):4021–4030. doi: 10.1172/JCI43670

Figure 7. Defective muscle regeneration in Dio2–/– mice.

Figure 7

(A) The percentage of centrally located nuclei was evaluated in H&E-stained TA muscle sections from D2KO and wild-type mice at 4–15 days after cardiotoxin (CTX) injection. (B) Number of nuclei was evaluated in H&E-stained TA muscle sections from Dio2–/– and wild-type mice at 15 days after CTX injection. *P < 0.05 compared with wild-type mice; n = 20. (C) Percentage of BrdU-labeled nuclei in TA muscle sections from Dio2–/– and wild-type mice at 15 days after CTX injection. *P < 0.05 compared with wild-type mice; n = 50 slides. Data are shown as average ± SD. (D) H&E-stained and DAPI-stained TA muscle sections (original magnification, ×40) from Dio2–/– and wild-type mice at 4 and 15 days after CTX injection. (E) Western blot analysis of total lysates from tibial muscles from 3 different mice at 15 days after CTX injection from the same experiment as in A. Tubulin was used as loading control. (F) The FoxO3/D2 axis promotes muscle differentiation in muscle cells by inducing MyoD expression.