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. Author manuscript; available in PMC: 2010 Oct 27.
Published in final edited form as: Immunity. 2009 Jul 17;31(1):60–71. doi: 10.1016/j.immuni.2009.05.010

Figure 4.

Figure 4

CDR2β swapping restores CD1d-glycolipid recognition. (A) The Vβ6 CDR2β was substituted with that of Vβ8.2, from positions 46 to 54 (boxed sequence). (B) Staining of hybridomas expressing the indicated TCRβ chain associated with the Vα14i TCRα chain. (C) Enzyme-linked immunosorbent assay of IL-2 production by hybridomas stimulated with mCD1d-expressing A20 cells in the presence of αGC (1 µg/ml), PBS57 (1 µg/ml) or iGb3 (10 µg/ml), or plate-bound anti-CD3 (5 µg/ml) and anti-CD28 (2 µg/ml) antibodies. Data represent the mean + s.e.m. of three independent experiments.