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. Author manuscript; available in PMC: 2010 Oct 27.
Published in final edited form as: Immunity. 2009 Jul 17;31(1):60–71. doi: 10.1016/j.immuni.2009.05.010

Figure 6.

Figure 6

CDR3β modulates iNKT TCR affinity. (A) Sequence of the wild-type Vβ8.2, wild-type Vβ7, wild-type Vβ6 WT and CDR2-modified Vβ6 CDR3β random constructs. (B) Staining of hybridomas expressing the indicated CDR3β random construct associated with the Vα14i TCRα chain. The percentage of αGC/CD1d tetramer+ cells is shown. Plots are gated on live eGFP+ cells and are representative of four independent experiments. (C) Enzyme-linked immunosorbent assay of IL-2 production by hybridomas stimulated with the indicated antigen in the presence of mCD1d-expressing A20 cells. One representative experiment out of two is shown.