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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: J Immunol. 2010 Mar 1;184(7):3866–3877. doi: 10.4049/jimmunol.0903417

FIGURE 1.

FIGURE 1

Effects of triptolide on IL-12 gene expression in LPS-treated MPM and MoDC (A–D) MPM (A, B), and MoDCs (C, D) were incubated for 15 h with different concentrations of triptolide and subsequently cultured for an additional 6 h in the presence (filled bars) or absence (open bars) of 1 μg/ml LPS. Mouse IL-12p40 (A), mouse IL-12p70 (B), human IL-12p40 (C), human IL-12p70 (D), and mouse IL-23 (E) levels in the supernatants were measured by ELISA. Experiment shown is representative of three to four independent experiments with mean ± SE. *p < 0.05. F–H, Total RNA was isolated from 1 × 106 MPM incubated with different concentrations of triptolide for 15 h with 1 μg/ml LPS (filled bars) or without (open bar) for an additional 3 h. Real-time PCR was performed to measure p40 (F), p35 (H) mRNA expression, and nascent transcripts for p40 (G). GAPDH mRNA was analyzed as an internal control. The experiment shown is representative of two independent experiments.