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. 2010 Oct 27;5(10):e13637. doi: 10.1371/journal.pone.0013637

Figure 3. Androgen Receptor ligands alter the expression of miRs in levator ani.

Figure 3

A and B. Sprague Dawley rats (n = 5; 200 g weight) were sham operated (Intact) and treated subcutaneously with vehicle or castrated and treated subcutaneously for 14 days with vehicle (ORX VEH (A); Cntrl Aver (B)), 3 mg/day SARM-1, SARM-2, an inactive SARM-3 or DHT. RNA from levator ani was extracted and the expression of 312 miRs were profiled and the miRs that are statistically different (P<0.05) from vehicle treated castrate animals are expressed in the heatmap. Values are expressed as an average with n = 5. (B). Clustering of individual samples are given in panel A. The numbers in bracket are numbers of animals. ORX vehicle in the heatmap represents pooled data from animals treated with vehicle and SARM-3. C. Ingenuity pathway analyses. The predicted targets of differentially expressed miRs were classified into major functional (left chart) and canonical (right chart) pathways using Ingenuity software. The bars represent pathways corresponding to the miR functional profiles of SARM-1, SARM-2 and DHT treated levator ani muscle in that respective order.