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. 2010 Oct 27;5(10):e13650. doi: 10.1371/journal.pone.0013650

Figure 4. Expression of ductal markers and inhibition of the ductal phenotype.

Figure 4

(A) RT-PCR for Cx43 and GSTπ (B) Western blotting analysis for Albumin, TFN, AFP and the liver enriched transcription factor C/EBPβ in control, EGF, Dex/EGF and Dex treated cells. β-actin and α-tubulin are also shown as loading controls. (C) Immunostaining for CK7 in control and Dex/EGF treated cells in the presence and absence and absence of the EGF receptor inhibitor AG1478. The inhibitor was added at a final concentration of 25 µM.