Figure 3. miR-146b, mir-155, miR-455 and miR-125a are located in annotated genes.

(A) The locations of the miRNA are shown relative to the genes in which they are contained. The structure of the genomic loci are shown, with coding exons shown by filled boxes and non-coding exons by open boxes. miR-155 is located in the exon of the non-protein coding BIC gene, while miR-146b and miR-455 are encoded in the introns of Tmem180 and Col27a1 respectively. miR-125a is located 5′ to the Ncrna00085 transcript. ESTs suggest that a further exon (hatched box), containing miR-125a, exists upstream of Ncrna00085. (B) BMDMs were stimulated with 100 ng/ml of LPS (upper panel) or 10⁶ cells/ml heat killed C. albicans (CA, lower panel) for the indicated times. Cells were then lysed and total RNA isolated as described in the methods. The levels of mRNA for Col27a1 were determined by Q-PCR. Error bars represent the standard deviation of stimulations from 4 independent cultures. (C) As (B) except that the levels of Tmem180 using primers spanning exons 3 to 4 were measured.