Figure 7.

Effects of Cl₂ exposure and DMTU treatment on markers of oxidative stress. (A) Nitric oxide was also measured 24 hours following Cl₂ exposure using a Griess reaction and no significant change was seen between any of groups. (B) Twenty-four hours following Cl₂ exposure BAL was collected and an OxyBlot was performed on lung tissue homogenates to detect carbonylated proteins. No significant differences were detected among the groups. (C) Twenty-four hours following chlorine exposure, lungs were collected for 4-HNE analysis. Chlorine caused a significant increase in 4-HNE levels over control and DMTU treated groups. There were no differences between DMTU groups and baseline. (n = 6-10, * p < 0.05).