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. 2010 Oct 27;5(10):e13676. doi: 10.1371/journal.pone.0013676

Figure 3. Treatment with cis-UCA (50 µg) maintains splenic T-regulatory cell populations.

Figure 3

129Sv/Ev mice were given 5% DSS for 7 days and treated with cis-UCA (50 µg) or PBS vehicle daily. Spleens from mice were removed on day 7. Total splenocytes were stained for CD4, CD25, FoxP3 and IL-10 and analyzed via flow cytometry. Cells positive for CD4 and CD25 (A) were gated and analyzed for their expression of FoxP3 (B). DSS treated mice had reduced levels of CD4+CD25+FoxP3+ cells when cell numbers were expressed as percentage of total cells (C). cis-UCA (50 µg) treated mice had similar numbers of CD4+CD25+FoxP3+ cells as compared with control mice (D). DSS and cis-UCA (50 µg) treatment did not alter levels of IL-10 secreting CD4+CD25+FoxP3+ cells (D). n = 4–13 for all measurements. a; p<0.05 DSS+PBS compared to control PBS, control UCA, and DSS+UCA (50 µg).