Table 1.
In vitro cytokine secretion into culture medium by human gestational membranes using a biopsy punch explant culture system or a transwell mounted explant culture systema
| Punch | Transwell Amnion | Transwell Choriodecidua | ||||
|---|---|---|---|---|---|---|
| Control | LPSb | Control | LPSb | Control | LPSb | |
| Interleukin-1β | 10.66 | 66.50 | 30.54 | 203.10 | 36.72 | 126.72 |
| (pg/ml) | (1.68) | (13.87) | (10.40) | (81.60) | (18.91) | (42.35) |
| Interleukin-6 | 4.42 | 78.83 | 15.22 | 80.34 | 23.75 | 104.19 |
| (ng/ml) | (1.24) | (20.27) | (5.33) | (30.10) | (7.33) | (23.81) |
| Interleukin-8 | 62.41 | 225.37 | 154.30c | 238.46 | 236.48c | 377.94 |
| (ng/ml) | (14.23) | (48.57) | (29.15) | (43.81) | (32.46) | (55.59) |
| Interleukin-10 | 12.61 | 335.11 | 15.42 | 166.57 | 15.34 | 290.66 |
| (pg/ml) | (2.78) | (50.05) | (4.21) | (69.59) | (4.13) | (70.21) |
| TNF-α | 21.37 | 5026.48 | 33.11 | 8394.66 | 50.04 | 11109.32 |
| (pg/ml) | (3.44) | (1419.42) | (4.31) | (4192.91) | (13.50) | (4789.19) |
Abbreviations: LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-α
a Cytokines were measured 12 h after incubation with or without 100 ng/ml LPS. In transwell cultures, LPS was added to both sides of the membranes and the cytokine concentrations are provided separately for the amniotic and choriodecidual compartments. Data are presented as mean (SEM). (N= 7 membranes)
b LPS treatments induced a significant increase of all cytokines compared with their respective controls (P < 0.05)
c Basal secretion of interleukin-8 differed significantly between the amniotic and choriodecidual sides of the transwell culture (P < 0.001)