Table 2.
Oligonucleotides used in this study
| Designation | Sequence (5'→3' direction) | Application |
|---|---|---|
| DIP0235-as | ctt ggt tgc cgg agc agc ctc ctt | PCR spaD |
| DIP0235-s | ccg aaa cca aga ccg aga aga ccg tca ag | PCR spaD |
| DIP0237-as | gca cac cag tca gcg cca agt cgc | PCR spaE |
| DIP0237-s | cgc gac tac gga acc gac acg ctg a | PCR spaE |
| DIP0238-as | gaa gtt gaa agg tcg gcc act aca gca a | PCR spaF |
| DIP0238-s | aaa ggg cta cta cat caa cat tcc aga cac | PCR spaF |
| DIP2010asT7 | cgc gta ata cga ctc act ata ggg tcc atc acg agg aac gac aac ggt ttt aga | spaC probe |
| DIP2010-s | cgc tac tcc tat ggg caa gca cct act gat att c | spaC probe |
| DIP2011asT7 | cgc gta ata cga ctc act ata ggg tgg tgg cga tgg cca gca gtc cga | spaB probe |
| DIP2011-s | tgc agc att cgc cga cga cca acc | spaB probe |
| SondeDIP2013-asT7 | ggg ccc taa tac gac tca cta tag gga gga ctg gag tgt tgc gcc g | spaA probe |
| SondeDIP2013-s | ggc gtc gaa aat caa gct gg | spaA probe |
| DIP2223asT7 | cgc gta ata cga ctc act ata ggg atc ggt aac ttc ctt acg gaa ctt ctc tgg cag | spaI probe |
| DIP2223-s | ttg ccc gcg gga act atc gac gga | spaI probe |
| SondeDIP2226-asT7 | ggg ccc taa tac gac tca cta tag ggc cca gcc cct gcg acg tc | spaH probe |
| SondeDIP2226-s | gga ggg ctg gga ggc agt ca | spaH probe |
| DIP2227asT7 | cgc gta ata cga ctc act ata ggg tcg acc ttg gac cag tgg acc tta gcg | spaG probe |
| DIP2227-s | ccg gac aga aga ttg ctg ccg agg ca | spaG probe |
| PromDIP2223-as | cgc ggg atc cag tag ggc gtc ctt tca gga | construction reporter plasmid for spaI |
| PromDIP2223-s | cgc gat gca tgt gac gcc attt tat gta cgc | construction reporter plasmid for spaI |
| PromDIP2226-as | cgc ggg atc cag ggt gtt ttc ctt tca gga | construction reporter plasmid for spaH |
| PromDIP2226-s | cgc gat gca tcg tca aag tta cgg ccg acc | construction reporter plasmid for spaH |
| PromDIP2227-as | cgc ggg atc cag tga aaa cac ctt cta ggg | construction reporter plasmid for spaG |
| PromDIP2227-s | cgc gat gca ttg aac cgg aat cat ttc tta | construction reporter plasmid for spaG |
| PromDIP2013-neu-as | cgc ggg atc ccc ctc aac tta ttt att tgg caa aaa g | construction reporter plasmid for spaA |
| PromDIP2013-neu-s | cgc gat gca tat tgg tga gac tac ttc ctt aaa gct ggt | construction reporter plasmid for spaA |