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. Author manuscript; available in PMC: 2010 Oct 27.
Published in final edited form as: J Inorg Biochem. 2007 Jun 12;101(10):1394–1399. doi: 10.1016/j.jinorgbio.2007.05.016

Fig. 3.

Fig. 3

Electrochemical oxidation of heme-rHO-1 complex under anaerobic conditions. (A) Absorption spectra of the heme-rHO-1 complex (50 μM) during the electrochemical-optical titration at 25 °C were recorded. The experimental conditions and procedures are described in the EXPERIMENTAL section. Applied potentials were: from thick solid line to dotted line, −0.395, −0.195, −0.145, −0.105, −0.075, −0.035, +0.005, and +0.205 V vs NHE. (B) The Nernst plot obtained from the absorbance change at 405 nm of the heme-rHO-1 complex.