Table 2. Primers for detection and genotyping of R. prowazekii into ancient teeth of Douai.

	PCR	Name	Primers	Tm	Sequence zise	Reference
Detection ofR. prowazekii	Real-time PCR	RpDet-F1RpDet-R2	5′-GTTGGATATATAAGGGTTTC-3′ 5′-CCGAGTCTATCTAATTTCCA-3 ′	55°C	206 bp	rpr_ORF0698 site-specific recombinases, DNA invertase Pin-like protein
	Nested PCR	RpDet-F3RpDet-R4	5′-ATGATCGTCAAGTGTTCGAT-3 ′ 5′-TAGACAGTCGCCATCTTGTA-3′	57°C	152 bp
Confirmation ofR. prowazekii	PCR standard	Rpro-F1Rpro-R1	5′-ACTGTTATTACCGATCTTGCCA-3′ 5′-TGGTTGATGCTAGGTTATTTGG-3′	58°C	187 bp	rpr_ORF0700, glutamine amidotransferase-like protein
	Nested PCR	Rpro-F11Rpro-R11	5′-GTATTAAGAATTTGATGCCACCA-3′ 5′-GTTATTAGTCCAAATGACGTGAA-3′	62°C	130 bp
R. prowazekiigenotyping	Real-time PCR	rpmE-F1rpmE-R2	5′-CCGGAAATGTAGTAAATCAATC-3′ 5′-CTGAGAATTTAAAGATTTATCTG-3 ′	59°C	210 bp	Yong Zhu et al., 2005 rpmE-tRNAfMet intergenic spacer
	Nested PCR	rpmE-F3rpmE-R4	5′-CTTTCGATAGCAAGAAAGAAGC-3 ′ 5′-CAGAGTATTAGTAGACGATACG 3′	62°C	115 bp