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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: J Dermatol Sci. 2010 Aug 10;60(1):29–39. doi: 10.1016/j.jdermsci.2010.07.015

Figure 2.

Figure 2

The Reported PKD Inhibitor, CID755673 Exhibited Low Potency to Inhibit Keratinocyte Proliferation or TPA-induced PKD Activation. (A) Near-confluent keratinocytes were treated with or without various concentrations of CID755673 as indicated for 24 hours prior to the addition of 1 µCi/mL [3H]thymidine for 60 minutes. [3H]Thymidine incorporation into DNA was then determined as in [15]. Values are expressed relative to the control (zero) and represent the means ± SEM of three independent experiments performed in duplicate; *p<0.05 versus the control. (B) Keratinocytes were pretreated for 2 hours with vehicle (DMSO, 0.1%) or 25 µM CID755673 prior to stimulation for 15 minutes with or without 100 nM TPA as indicated. Cells were harvested and subjected to western analysis as described in Methods. This experiment was repeated twice with similar results. Comparable data were obtained in three separate experiments performed with 50 µM CID755673 (data not shown).