Figure 8.

The Protein Kinase C/PKD Inhibitor, Gö6976, Inhibited the Late TPA-induced Proliferation but not the Initial TPA-elicited Inhibition of Proliferation, as well as the Late TPA-induced Stimulation of Transglutaminase Activity but not the Initial TPA-elicited Effect. (A) Near-confluent keratinocytes were treated in the presence and absence of 100 nM TPA with and without the various inhibitors, as shown, for the indicated times prior to the addition of 1 µCi/mL [³H]thymidine for 60 minutes. The cells were then harvested and DNA specific activity was determined as in [13]. Values represent the means ± SEM of 3 independent experiments performed in duplicate; *p<0.01, **p<0.001 versus the control; +p<0.001 versus TPA alone. (B) Near-confluent keratinocytes treated in the presence and absence of 100 nM TPA with and without the various inhibitors, as shown, for the indicated times were harvested and transglutaminase activity was determined as in [13]. Values represent the means ± SEM of 3 independent experiments performed in duplicate; *p<0.01, **p<0.001 versus the control; +p<0.01 versus TPA alone.