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. 2010 Aug 10;16(11):3507–3514. doi: 10.1089/ten.tea.2010.0097

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Copyright 2010, Mary Ann Liebert, Inc.

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FIG. 1. — Derivation of mesenchymal stem cells (MSCs) from human embryonic stem cell (hESC) culture. Phase-contrast microscopy image of 10-day embryoid body formed after hESCs was transferred to low attachment Petri dishes (A). After 1 week of culture on gelatin-coated tissue culture plates, heterogeneous cells migrated out from embryoid body (B). After 2 weeks of culture, more MSC-like cells emerged at the edge (C). The cells were enzymatically dissociated and expanded to passage 4 to form a homogeneous MSC population (D). Immunofluorescence stain of CD73 (green) and counterstain of nuclei with 4′-6-diamidino-2-phenylindole (blue) showed that the cells were CD73 positive (E). Scale bars: 100 μm. Color images available online at www.liebertonline.com/ten.

FIG. 1. — Derivation of mesenchymal stem cells (MSCs) from human embryonic stem cell (hESC) culture. Phase-contrast microscopy image of 10-day embryoid body formed after hESCs was transferred to low attachment Petri dishes (A). After 1 week of culture on gelatin-coated tissue culture plates, heterogeneous cells migrated out from embryoid body (B). After 2 weeks of culture, more MSC-like cells emerged at the edge (C). The cells were enzymatically dissociated and expanded to passage 4 to form a homogeneous MSC population (D). Immunofluorescence stain of CD73 (green) and counterstain of nuclei with 4′-6-diamidino-2-phenylindole (blue) showed that the cells were CD73 positive (E). Scale bars: 100 μm. Color images available online at www.liebertonline.com/ten.