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. 2010 Aug 10;16(11):3507–3514. doi: 10.1089/ten.tea.2010.0097

FIG. 5.

FIG. 5.

Three-dimensional osteogenesis of hESC-MSCs on polylactic acid NF scaffolds. hESC-MSCs were seeded into scaffolds and cultured in the control basic medium or basic medium supplemented with Dex/BMP-7 for 6 weeks. Samples were collected for histological analysis, calcium content quantification, and gene expression analysis. Hematoxylin and eosin stain showed that cells grew throughout the scaffold for constructs cultured in the control basic medium (A) or Dex/BMP-7-supplemented medium (B). von Kossa stain showed that there was no mineralization for cultures in the control basic medium (C), but highly mineralized in constructs cultured with Dex/BMP-7 (D). Calcium content quantification confirmed that Dex/BMP-7 treatment promoted calcium deposition into constructs after 6 weeks of culture (E). RT-polymerase chain reaction analysis further demonstrated that expression of bone-specific marker genes, bone sialoprotein (BSP) and osteocalcin (OCN), were increased for constructs cultured with Dex/BMP-7 (F). Scale bars: 100 μm. *p < 0.05. Color images available online at www.liebertonline.com/ten.