FIG. 3.

Effect of initial ES cell concentration on size distribution of EBs in static culture at varying cell densities (A), spinner flask at varying cell seeding densities (B) and speeds (C), and Synthecon at varying cell seeding densities (D) and speeds (E). EBs were sorted into bins of 50 μm with a polynomial trendline shown. In general, the static system (A) showed least control over EB size, as demonstrated by the larger peak width. In the Synthecon system, higher rotation speeds demonstrate a trend of smaller EBs compared to lower rotation speeds (E). Color images available online at www.liebertonline.com/ten.