Figure 10.
Western blot analysis of photolabeling of Rce1p with probe 6 detected with anti-HA following SA pull-down and SDS-PAGE separation. Lane 1: Rce1p-containing membranes (RCE1) and 6 (15 μM), no UV irradiation. Lane 2: Membranes lacking Rce1p (rce1Δ) and 6 (15 μM), no UV irradiation. Lane 3: Rce1p-containing membranes (RCE1) and 6 (15 μM), with UV irradiation. Lane 4: Membranes lacking Rce1p (rce1Δ) and 6 (15 μM), with UV irradiation. Results shown are representative of those obtained in three independent experiments.