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. 2010 May 28;38(19):6526–6532. doi: 10.1093/nar/gkq434

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Dependence of the DNA elongation on the staining ratio R_s. (a) Fractional elongation γ, defined as the increase in contour length due to labeling normalized by the length of the unlabeled molecule, plotted over R_s (open circles). Data were fit with a simple two-line model assuming an initial linear increase below the critical staining ratio followed by a saturation at γ_sat (orange solid line). This provided = 0.31±0.06 dye/bp and γ_sat = 0.47±0.02. (b) Experimental data from (a) with fits (lines) to the model of McGhee and von Hippel [Equation (2)] for different association constants K_a. Fitting results are summarized in Table 1. The data were best fit (orange dashed line) for K_a = 1.4 × 10⁹ M⁻¹, = 0.31 dye/bp and γ_sat = 0.50.

Inline graphic — Dependence of the DNA elongation on the staining ratio R_s. (a) Fractional elongation γ, defined as the increase in contour length due to labeling normalized by the length of the unlabeled molecule, plotted over R_s (open circles). Data were fit with a simple two-line model assuming an initial linear increase below the critical staining ratio followed by a saturation at γ_sat (orange solid line). This provided = 0.31±0.06 dye/bp and γ_sat = 0.47±0.02. (b) Experimental data from (a) with fits (lines) to the model of McGhee and von Hippel [Equation (2)] for different association constants K_a. Fitting results are summarized in Table 1. The data were best fit (orange dashed line) for K_a = 1.4 × 10⁹ M⁻¹, = 0.31 dye/bp and γ_sat = 0.50.