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. 2010 Jun 4;38(19):6533–6543. doi: 10.1093/nar/gkq451

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Analysis of MTase activities of S. cerevisiae Trm10p, S. acidocaldarius Saci_1677p and T. kodakaraensis TK0422p on total (bulk) tRNA from the S. cerevisiae Y16243 strain, in which the TRM10 gene is inactivated. tRNA was incubated in presence of [methyl-¹⁴C]AdoMet and purified Trm10p (A), Saci_1677p (B) and TK0422p (C) as described in ‘Materials and Methods’ section. After incubation, tRNA was recovered and digested by nuclease P1. The resulting nucleotides were analysed by 2D–TLC and autoradiography. Circles in dotted lines show the migration of the four canonical nucleotides used as UV markers.

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