Figure 2.

Analysis of MTase activities of S. acidocaldarius Saci_1677p and T. kodakaraensis TK0422p on total E. coli tRNA. E. coli tRNA was incubated in presence of 5 µg of purified Saci_1677p (A) or TK0422p (B) and [methyl-¹⁴C]AdoMet (see ‘Materials and Methods’ section). tRNA was recovered and digested by nuclease P1. The resulting 5′-phosphate mononucleosides were analysed by 2D–TLC followed by autoradiography.