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Thermococcus kodakaraensis TK0422p MTase displays tRNA (m1A and m1G) MTase activity in E. coli in vivo. tRNA from the E. coli strain expressing (C) or not (B) TK0422p were extracted and hydrolysed by nuclease P1, snake venom phosphodiesterase and alkaline phosphatase, and then analysed by HPLC. The peaks corresponding to m1A and m1G are indicated by arrows. A standard curve with the reference nucleosides is given in (A).
