Table 1.
pH dependence of m1A and m1G formation by TK0422p from T. kodakaraensis
| pH | m1G/m1A ratio |
|---|---|
| 4.8 | 0 |
| 5.5 | 0.06 |
| 7 | 0.42 |
| 9 | 0.18 |
| 9.75 | 1.43 |
The MTase activity was measured using total (bulk) E. coli tRNA as substrate. tRNA was incubated in presence of [methyl-14C]AdoMet and purified TK0422p protein. The different buffers are described in ‘Materials and Methods’ section. After incubation, tRNA was recovered and the formation of m1G and m1A was measured as described in ‘Materials and Methods’ section.