Figure 6.
Nuclear translocation of TREX1. (A) Exponentially growing wt MEFs were non-exposed or exposed to 20 J/m2 UV for 3 and 6 h. Nuclear and cytoplasmic extracts were isolated. Immunodetection was performed using TREX1, PCNA, GAPDH or, as loading control, β-Actin-specific antibody. Induction factor (IF) is derived from densitometric measurement of TREX1 signal and normalized to β-Actin expression. (B) Exponentially growing wt MEFs were not exposed (control) or exposed to 20 J/m2 UV light or 2.5 µM B(a)P for 6 h and thereafter fixed as described. TREX1 localization was visualized by the use of a specific antibody and detected by confocal laser scanning microscopy.