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. 2010 May 28;38(19):6418–6432. doi: 10.1093/nar/gkq455

Figure 7.

Figure 7.

Impact of TREX1 on DNA replication. (A) Exponentially growing TREX1 wt cells (sc14+/+) and TREX1-deficient cells (sc3−/− and sc8−/−) were exposed to 7.5 J/m2 UV (left panel) or 2.5 µM B(a)P (right panel). Different time points later DNA replication was measured by incorporation of BrdU added to the medium 1 h before harvest. Data are the mean of three independent experiments +/− SD. *P < 0.05, **P < 0.01, ***P < 0.001. (B) Exponentially growing wt MEFs were exposed to 20 J/m2 UV light for 6 h and thereafter fixed as described. TREX1 and PCNA co-localization was analysed by the use of a specific antibody and detected by confocal laser scanning microscopy. (C) Total cell extracts were isolated from exponentially growing wt MEFs irradiated with 20 J/m2 UV or exposed to 2.5 µM B(a)P for 6 h. When indicated, RNase-free DNase I was added to the extracts at 1 U/µl for 1 h at 32°C prior to IP of TREX1 utilizing the Catch and Release® v2.0 system from Millipore. Co-immunoprecipitated PCNA was visualized as described above.