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Lack of endonucleolytic activity in MeP-half-site reactions of Cre(R173A, Y324G). Reactions and product analysis were performed as described under Figure 2. The results of SDS–PAGE and denaturing PAGE analyses are shown in A and B, respectively. The predicted positions of migration of the covalent intermediate and the hydrolysis product (HP) are indicated. The incubation time for the control reaction without addition of Cre(R173A, Y324G) was 24 h.
