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Lack of strand cleavage or hydrolysis in a P-half-site by Cre(R173A). The half-site substrate was identical in sequence to that employed in the reactions shown in Figures 2 and 3, except that it contained a scissile phosphate instead of methylphosphonate. Reactions were analyzed as described in the legends for Figures 2 and 3. (A) SDS-PAGE. (B) Urea-PAGE.
