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The N-terminal effector domain (NED) does not interact with AGO1. Drosophila S2 cells were transfected with plasmids encoding the full-length NHA-dGW182 or its deletion mutants. Cell lysates were used in immunoprecipitations with anti-AGO1 antibody. Inputs and immunoprecipitates were analyzed by western blotting using anti-HA or anti-AGO1 antibodies, as indicated on the left.
