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Repression mediated by the NED is independent of the poly(A) tail. Drosophila S2 cells were transfected with the boxB-containing FLuc reporters, either FLuc-boxB (open bars) or FLuc-boxB-HSL (black bars). Unlike FLuc-boxB, FLuc-boxB-HSL lacks a polyadenylation signal and bears instead a histone H1 stem-loop (HSL) structure in its 3′UTR. FLuc reporters were co-transfected with RLuc as a normalization control, and plasmids encoding indicated NHA-fusion proteins.
