Figure 7.

N-terminal domains of human GW182 homologs, TNRC6A, B and C, are able to repress tethered mRNA in Drosophila S2 cells. The assay was performed as in Figure 1. (A) The N-terminal fragments of human TNRC6A (Q8NDV7; amino acids 688–1096), TNRC6B (Q9UPQ9, amino acids 589–921), and TNRC6C (Q9HCJ0, amino acids 417–848) aligning with the Drosophila NED and enriched in GW-rich repeats (see Figure 3 and Supplementary Figure S1) were tethered to the FLuc-boxB reporter. dGW182 was tethered as a positive control, NHA-lacZ as the negative control. (B) Expression levels of NHA-fusion proteins were estimated by western blotting with anti-HA antibody.