Table 1.
Plasmid constructs used
| Amino acid Substitution | pT7-7 derivative | pET22b derivative | pET22b-cat derivative |
|---|---|---|---|
| Wild-type | pGS583 | pYJ001 | pAM447 |
| R118A | pGB049 | pGB050 | pAM450 |
| R118C | pGB045 | pGB047 | pAM441 |
| F120S | pGB051 | pGB052 | pAM465 |
| F120T | pGB053 | pGB054 | pAM466 |
| R118C, F120T | pGB065 | pYJ019 | |
| Q212A | pYJ017 | pYJ020 | |
| E218R | pYJ013 | pAM448 | |
| H222A | pYJ003 | pYJ006 | pAM443 |
| K227A | pYJ007 | pAM444 | |
| F120T, K227A | pGB084 | ||
| Δfinger (P76G, Δ77–115, L116T) | pGB043 | pAM434 | pAM437 |
| Δfinger, R118C | pGB046 | pGB048 | pAM442 |
| Fingertip (R97S, K98Q, K100Q, K101E) | pGB055 | pGB056 | pAM468 |