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. 2010 Jun 20;38(19):6577–6588. doi: 10.1093/nar/gkq527

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Measurement of de novo transcription. (A) In organello transcription of 16-week-old rescue and control hearts, followed by normalization by Northern blotting for the mitochondrial transcripts of ND6. (B) Schematic diagram of the murine mtDNA control region containing tRNA^T (T), tRNA^P (P), TAS 1 and 2, 7S RNA transcript, 7S DNA fragment, conserved sequence blocks 1–3 (CSB), LSP, HSP and tRNA^F (F). (C) S1 protection assay of LSP proximal transcription (7S RNA) and control transcript (ND6) of control (C) and rescue (R) mitochondrial RNA extracts. (D and E) Relative transcript levels normalized to the nuclear 18S rRNA transcript. Levels of the mitochondrial transcripts of COX1 (light grey bars) and ND6 (dark grey bars) and the large mitochondrial ribosomal subunit 16S rRNA (white bars) are shown at 12 (D) and 52 weeks (E). Data are presented as means ± SEM percentage of controls; *P < 0.05; ***P < 0.001, respectively.