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. 2010 Jun 11;38(19):6544–6554. doi: 10.1093/nar/gkq536

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Determination of mutual interaction regions in HDM2 and RPL26. (A) A diagram for the deletion mutants of HDM2 is shown. (B) Mapping of the HDM2 domain for RPL26 binding. Extracts from HEK293 cells transfected with the indicated plasmid DNA encoding deletion mutants of HDM2 and Flag-RPL26 were immunoprecipitated with Myc antibody, and followed by immunoblotting with Flag or Myc antibodies. (C) A diagram for the deletion mutants of RPL26 is shown. (D) Mapping of the RPL26 domain for HDM2 binding. Extracts from HEK293 cells transfected with the indicated plasmid DNA encoding deletion mutants of RPL26 and HA-HDM2 were immunoprecipitated with HA antibody, and followed by immunoblotting with HA and Myc antibodies.