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. Author manuscript; available in PMC: 2011 Dec 15.
Published in final edited form as: Arch Biochem Biophys. 2010 Sep 15;504(2):169–176. doi: 10.1016/j.abb.2010.08.021

Figure 3. Simulated progress curves for pentasaccharide binding to native and latent antithrombins by two-step and three-step induced-fit binding mechanisms.

Figure 3

Simulations of pentasaccharide binding to native and latent antithrombins by the two-step mechanism of scheme 1 or the three-step mechanism of scheme 2 were generated by numerical integration of the differential rate equations for the binding models using the kinetic constants in table 3. A series of binding progress curves were calculated for an antithrombin concentration of 0.025 μM and pentasaccharide concentrations ranging from 0.25–15 μM (circles) to duplicate the pseudo-first order experimental conditions and pentasaccharide concentrations used to measure the binding kinetics. Progress curves were fit by a single exponential function (solid lines) to obtain the observed pseudo-first order binding rate constant.