Table 1. Cardiac and skeletal muscle phenotypes at 2 dpf in embryos injected with morpholinos targeting the three zebrafish troponin C genes.

Embryos were injected at the one- to four-cell stage with tnnc1a, tnnc1b, or tnnc2 morpholinos either singly or in pair-wise fashion (see Methods). Embryos were then assayed for (A) heart or (B) skeletal muscle contraction at 2 dpf and were characterized as one of the following phenotypes; wild-type (contraction of both chambers with blood circulation); sil phenotype (contraction of the atrium, silent ventricle, and absence of blood circulation); contraction, no flow (contraction of both chambers with the absence of blood circulation); no contraction, no flow (no contraction in either chamber with the absence of blood circulation); sk. m. impaired (decreased touch-response); paralyzed (no touch response).

Table 1A. Cardiac phenotype at 2 dpf in embryos injected with morpholinos targeting three zebrafish troponin C genes.
	wild-type	sil phenotype	contraction no flow	no contraction no flow
uninjected	316 (99.7%)	0 (0%)	1 (0.3%)	0 (0%)
tnnc1a	1 (0.7%)	150 (98.6%)	1 (0.7%)	0 (0%)
tnnc1b	134 (75.3%)	0 (0%)	43 (24.1%)	1 (0.6%)
tnnc2	137 (99.3%)	0 (0%)	1 (0.7%)	0 (0%)
tnnc1a + tnnc1b	0 (0%)	2 (2.3%)	0 (0%)	84 (97.7%)
tnnc1a + tnnc2	0 (0%)	74 (100%)	0 (0%)	0 (0%)
tnnc1b + tnnc2	51 (77.3%)	0 (0%)	15 (22.7%)	0 (0%)

Table 1B. Skeletal muscle phenotype at 2 dpf in embryos injected with morpholinos targeting three zebrafish troponin C genes.
	wild-type	sk. m. impaired	paralyzed
uninjected	317 (100%)	0 (0%)	0 (0%)
tnnc1a	152 (100%)	0 (0%)	0 (0%)
tnnc1b	0 (0%)	178 (100%)	0 (0%)
tnnc2	0 (0%)	138 (100%)	0 (0%)
tnnc1a + tnnc1b	0 (0%)	86 (100%)	0 (0%)
tnnc1a + tnnc2	0 (0%)	31 (42%)	43(58%)
tnnc1b + tnnc2	0 (0%)	0 (0%)	66(100%)