Fig. 3.

Dicer siRNA causes a various types of spindle and chromosome disorganizations. Oocytes in negative control and Dicer siRNA groups were fixed with 4% parafirnaldehyde and stained with alphe tubulin immunofluroscence staining (green) and DNA was counterstained with DAPI (red). Representative images are shown. Bar, 25 um. a normal spindle and chromosomal organization. Oocytes exhibited single barrel-shaped MI spindles with chromosomes congregated at the metaphase plate. b abnormal spindle organization. Oocytes exhibited irregularly dispersed spindle microtubules. c chromosomal misaligned. Oocytes exhibited chromosomes scattering away from the metaphase plate. d abnormal spindle organization plus chromosomal misalignment. Oocytes were with irregularly dispersed spindle microtubules and with chromosomes scattered away from metaphase plate