Fig. 5.

Effect of Dicer siRNA can be rescued by ooplasmic replacement. Ooplasmic replacement was performed as described in text. The experiments were performed three times and data are presented as mean+S.D. a shows the maturation rates of Dicer siRNA, negative control, and non-injected control. b shows relative mRNA of Dicer in negative control, Dicer siRNA and ooplasmic replacement. c shows the identification of amplicons of real-time RT-PCR by gel electrophoresis and beta actin is used as internal control. Data in a were analyzed by a Pearson’s Chi square test. P < 0.05 was considered statistically significant. The maturation rates (judged by percentages of oocyte at the MII stage) were not significantly different among the three studied groups. P = 0.333